Recombinant Mouse Glypican-4 (Gpc4), partial

SKU: P51655

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: P51655

Gene Names: Gpc4

Alternative Name(s): K-glypican

Abbreviation: Recombinant Mouse Gpc4 protein, partial

Organism: Mus musculus (Mouse)

Source: E.coli

Expression Region: 530-557aa

Protein Length: Partial

Tag Info: N-terminal 6xHis-SUMO-tagged

Target Protein Sequence: AGGAHAEAKPYLLAALCILFLAVQGEWR

MW: 19 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous syst.

Reference: K-glypican a novel GPI-anchored heparan sulfate proteoglycan that is highly expressed in developing brain and kidney.Watanabe K., Yamada H., Yamaguchi Y.J. Cell Biol. 130: 1207-1218(1995)

Function: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system.