Recombinant Mouse G-CSF protein

SKU: CD00437

Size:2 ug

Background:Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNFα and IFN-γ. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL1 or TNF-α activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Granulocyte Colony Stimulating Factor

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse NFS-6 cells is less than .5 ng/ml, corresponding to a specific activity of > 2. × 17 IU/mg.

Synonyms:CSF3,Csf, Csfg, G-CS, G-CSF, MGI-, MGI-IG, Mouse

Endotoxin:Less than 0.1 EU/μg of rMuG-CSF as determined by LAL method.

Storage:-20ºC

Sequence:VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA

uniprot accession: P992

Molecular weight:Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 178 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 1 mM Sodium Citrate, pH 4., 15 mM NaCl, .1 % Tween-2.