Recombinant Mouse EphB4/HTK Protein (Fc Tag)

SKU: PKSM040599

Size: 200μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: AI042935;Htk;MDK2;Myk1;Tyro11

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-hFc

UNIProt ID: P54761

Accession: P54761-1

Background: Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.

Concentration:

Activity: Immobilized mouse EPHB2-His at 10 μg/mL (100 μl/well) can bind mouse EPHB4-Fc, The EC50 of mouse EPHB4-Fc is 15 ng/mL.

Sequence: Met 1-Ala 539

Purity: > 97 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 84.7 kDa

ObservedMW: 110 kDa