Recombinant Mouse CD32/FCGR2B Protein (His & AVI Tag), Biotinylated

SKU: PKSM040900

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: AI528646;CD32;F630109E10Rik;Fc[g]RII;FcgRII;Fcgr2;Fcgr2a;Fcr-2;Fcr-3;Ly-17;Ly-m20;LyM-1;fcRII

Research Areas:

Conjugation: Biotinylated

Target Species: Mouse

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-His-Avi

UNIProt ID:

Accession: NP_001070657.1

Background: Receptors for Fc portion of IgG (Fcγ Rs) are members of the Ig superfamily, and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). CD32 protein is a low affinity receptor for IgG that binds only IgG immune complexes and is expressed on a diverse range of cells such as monocytes, macrophages, neutrophils, eosinophils, platelets, and B cells. Human CD32 class is encoded by three closely related genes, and designated Fcγ RII A, B, and C which share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32 is involved in a number of immune responses including antibody-dependent cell-mediated cytotoxicity, clearance of immune complexes, release of inflammatory mediators, and regulation of antibody production.

Concentration:

Activity: 1. Measured by its ability to bind human IgG1 in a functional ELISA. 2. Labeling ratio of biotin to protein: 0.5

Sequence: Met 1-Arg 217

Purity: > 97 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 23.9 kDa

ObservedMW: 38-43 kDa