Recombinant Mouse CD20/MS4A1 protein (His tag)

SKU: PDEM100311

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:CD20;MS4A1

Target Synonym:B-cell differentiation antigen Ly-44;B-lymphocyte antigen CD20;CD20;Lymphocyte antigen 44;Membrane-spanning 4-domains subfamily A member 1;Ms4a1

Research Areas:Cancer; Immunolegy;Neuroscience; Stem Cells;Epigenetics and Nuclear signaling.

Conjugation:

Target Species:Mouse

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:P19437

Accession:P19437

Background:CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.

Concentration:

Activity:Not validated for activity

Sequence:Leu 133-Pro 291

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:17.4 kDa

ObservedMW:28 kDa