Recombinant Lactobacillus salivarius Enolase (eno)

SKU: Q1WSY0

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: Q1WSY0

Gene Names: eno

Alternative Name(s): (2-phospho-D-glycerate hydro-lyase)(2-phosphoglycerate dehydratase)

Abbreviation: Recombinant Ligilactobacillus salivarius Enolase protein

Organism: Ligilactobacillus salivarius (strain UCC118) (Lactobacillus salivarius)

Source: E.coli

Expression Region: 1-441aa

Protein Length: Full Length

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein Sequence: MSAITEIYAREVLDSRGNPTVEAEVYTQDGGFGRGIVPSGASTGEHEAVELRDGDKDRYMGKGVTKAVANVNDIIAKEIVGYEVTDQVAIDKAMIALDGTPNKGKLGANAILAVSIAVARAAADELQVPLYNYIGGFNAHVLPTPMMNVINGGAHSDNKVDFQEFMIMPVGAPTVKEAIRMGSETFHHLQKLLAADGKVTSVGDEGGFAPDFANNEEPLEYLIKAIEAAGYKPGKDIAIAVDVASSELWDNDSKKYKLRWSTGEEFTTEEFIKYLEGLVAKYPIISIEDPIDENNWDDWVTITSELGKKVQLVGDDFFVTNTEYLRKGIKMGAANSILIKVNQIGTLTETMEAIEMAKEAGYTAIVSHRSGETEDTTIADLVVATNAGQIKTGSMSRTDRLAKYNQLMRIEDQLTDLVAKYKGINSFYNLSEQARQDIINK

MW: 55.5 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.

Reference: "Multireplicon genome architecture of Lactobacillus salivarius." Claesson M.J., Li Y., Leahy S., Canchaya C., van Pijkeren J.P., Cerdeno-Tarraga A.M., Parkhill J., Flynn S., O'Sullivan G.C., Collins J.K., Higgins D., Shanahan F., Fitzgerald G.F., van Sinderen D., O'Toole P.W. Proc. Natl. Acad. Sci. U.S.A. 103: 6718-6723(2006)

Function: