Recombinant Human Wnt3a surrogate (Active)

SKU: N/A

Size: 100ug. Other sizes are also available.

Activity: Yes

Research Areas: N/A

Uniprot ID: N/A

Gene Names: Wnt3a surrogate

Alternative Name(s): /

Abbreviation: Recombinant Human Wnt3a surrogate protein (Active)

Organism: Homo sapiens (Human)

Source: Mammalian cell

Expression Region: 1-306aa

Protein Length: Full Length

Tag Info: C-terminal hFc1-tagged

Target Protein Sequence: N/A

MW: 58.5 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Less than 1.0 EU/μg as determined by LAL method.

Biological_Activity: Measured by its ability to induce Topflash reporter activity in HEK293T cells. The ED50 for this effect is 17.76-26.50 ng/mL.

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 μm sterile filtered 25 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.2

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

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