Recombinant Human WDYHV1/NTAQ1 Protein (GST Tag)

SKU: PKSH032966

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: WDYHV1;NTAQ1

Target Synonym: C8orf32;N-terminal Gln amidase;NTAQ1;Nt(Q)-amidase;Protein N-terminal glutamine amidohydrolase;Protein NH2-terminal glutamine deamidase;WDYHV1

Research Areas: Cell biology

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-GST

UNIProt ID: Q96HA8

Accession: AAH08781.1

Background: Human protein N-terminal glutamine amidohydrolase (WDYHV1) is an enzyme that in humans is encoded by the WDYHV1 gene, belongs to the NTAQ1 family.WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, which is an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. However,it does not act on substrates with internal or C-terminal glutamine andnon-glutamine residues in any position. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Cys205

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of PBS,100mM GSH,1% TritonX-100,15% Glycerol,pH7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 49.8 kDa

ObservedMW: 45-50 kDa