GeneBio Systems
Recombinant Human TPBG (C-6His)
Recombinant Human TPBG (C-6His)
SKU:EPT261
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Size:50ug
Description:Recombinant Human Trophoblast Glycoprotein is produced by our Mammalian expression system and the target gene encoding Ser32-Ser355 is expressed with a 6His tag at the C-terminus.
Accession:Q13641
Molecular weight:36.1 KDa
Apparent molecular weight:55-80 KDa, reducing conditions
Other names:5T4 Protein, Human;5T4AG Protein, Human;M6P1 Protein, Human;WAIF1 Protein, Human
Storage condition:Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity:Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Biological activity:
Redissolve:Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage:Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Delivery condition:The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Background:Trophoblast glycoprotein, also known as TPBG and 5T4, it belongs to the LRR family of proteins. The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. It expressed by all types of trophoblasts as early as 9 weeks of development. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
