Recombinant Human Pro-neuregulin-1, membrane-bound isoform (NRG1), partial (Active)

SKU: Q02297

Size: 100ug. Other sizes are also available.

Activity: Yes

Research Areas: Others

Uniprot ID: Q02297

Gene Names: NRG1

Alternative Name(s): Pro-neuregulin-1; Neuregulin-1 beta 1; NRG1-beta 1; HRG1-beta 1; EGF;NRG1; GGF; HGL; HRGA; NDF; SMDF

Abbreviation: Recombinant Human NRG1 protein, partial (Active)

Organism: Homo sapiens (Human)

Source: Mammalian cell

Expression Region: 20-242aa

Protein Length: Partial

Tag Info: Tag free

Target Protein Sequence: SGKKPESAAGSQSPALPPRLKEMKSQESAAGSKLVLRCETSSEYSSLRFKWFKNGNELNRKNKPQNIKIQKKPGKSELRINKASLADSGEYMCKVISKLGNDSASANITIVESNEIITGMPASTEGAYVSSESPIRISVSTEGANTSSSTSTSTTGTSHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCQPGFTGARCTENVPMKVQNQEKAEELYQKR

MW: 24.2 kDa

Purity: Greater than 95% as determined by SDS-PAGE.

Endotoxin: ≤10 EU/mg by the LAL method

Biological_Activity: Measured in a cell proliferation assay using MCF-7 breast cancer cell line. The ED50 for this effect is 1-8 ng/mL.

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 μm filtered solution containing PBS, 5% mannitoland 0.01% Tween 80, pH 7.4

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

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