Recombinant Human PDGFRa/CD140a Protein (Fc Tag)

SKU: PKSH031529

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: Alpha platelet-derived growth factor receptor;CD140 antigen-like family member A;CD140A;PDGFR-2;PDGFR-alpha;PDGFR2;Platelet-derived growth factor alpha receptor;Platelet-derived growth factor recepto;Platelet-derived growth factor receptor alpha;RHEPDGFRA

Research Areas: Signal Transduction;Cardiovascular;Cancer;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-hFc

UNIProt ID: P16234

Accession: NP_006197.1

Background: PDGFRA; also known as CD140a; together with the structurally homolog protein PDGFRB (CD140b); are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell; and binds all three ligand isoforms PDGF-AA; PDGF-BB and PDGF-AB with high affinity; whereas PDGFRB dose not bind PDGF-AA. PDGFRA plays an essential role in regulating proliferation; chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation; and in addition; particularly important for kidney development. Additionally; PDGFRA is involved in tumor angiogenesis and maintenance of the tumor microenvironment and has been implicated in development and metastasis of Hepatocellular carcinoma (HCC). PDGFRA may represent a potential therapeutic target in thymic tumours. PDGFRA gene amplification rather than gene mutation may be the underlying genetic mechanism driving PDGFRA overexpression in a portion of gliomas.

Concentration:

Activity: 1. Measured by its ability to bind human PDGF-C in a functional ELISA. 2. Measured by its ability to bind mouse PDGF-C in a functional ELISA.

Sequence: Met 1-Glu 524

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 82.9 kDa

ObservedMW: 120-130 kDa