Recombinant Human NEF3 protein (GST tag)

SKU: PDEH100782

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:NEF3

Target Synonym:160 kDa neurofilament protein;NEF3;NEFM;NF-M;NFM;Neurofilament 3;Neurofilament medium polypeptide;Neurofilament triplet M protein

Research Areas:Neuroscience

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-GST

UNIProt ID:P07197

Accession:P07197

Background:Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.

Concentration:

Activity:Not validated for activity

Sequence:Ser 2-Ser 358

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:64.2 kDa

ObservedMW:60 kDa