Recombinant Human LCP2 Protein (His Tag, N-T7 Tag)

SKU: PKSH032716

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: LCP2

Target Synonym: LCP2;Lymphocyte cytosolic protein 2;SH2 domain-containing leukocyte protein of 76 kDa;SLP-76 tyrosine phosphoprotein;SLP76

Research Areas: Signal Transduction;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-T7 & C-His

UNIProt ID: Q13094

Accession: Q13094

Background: Lymphocyte cytosolic protein 2（LCP2）contains a SAM domain and a SH2 domain. It is highly expressed in spleen, thymus and peripheral blood leukocytes, T-cell and monocytic cell lines, but expressed at lower level in B-cell lines. LCP2 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. It is phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2 during post-translational modification. Studies using LCP2-deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Pro533

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, 20% Glycerol, pH 8.5.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 62.6 kDa

ObservedMW: 70 kDa