Recombinant Human LAG3/CD223 Protein (Fc Tag)

SKU: PKSH033597

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Immune Checkpoint Proteins

Abbreviation: LAG3;CD223

Target Synonym: CD223;LAG-3;LAG3;Lymphocyte activation gene 3 protein;Protein FDC

Research Areas: immunology

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-Fc

UNIProt ID: P18627

Accession: P18627

Background: Human Lymphocyte activation gene 3 protein( LAG3) is a member of immunoglobulin (Ig) superfamily. LAG3 contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. LAG3 is involved in lymphocyte activation and can bind to HLA class-II antigens. It is selectively expressed in activated T and NK cells. LAG3 has a negative regulatory function in T cells and acts as as a new marker of T cell induced B cell activation. As a soluble molecule; LAG3 activates antigen-presenting cells through MHC class II signaling. It can lead to increased antigen-specific T-cell responses in vivo. LAG-3 has higher affinity to MHC class II than CD4.

Concentration:

Activity: Immobilized Anti-Human LAG-3 mAb at 2μg/ml (100 μl/well)can bind Human LAG-3-Fc. The ED₅₀ of Human LAG-3-Fc is 6.76 ng/ml.

Sequence: Leu23-Leu450

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM NaH₂PO₄, 150mM NaCl, 0.1M Arginine, 0.1M Glu, 10% Glycerol, 0.01% Tween20, 5% Trehalose, pH 7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 73.3 kDa

ObservedMW: 70-90 kDa