Recombinant Human IL-3 protein

SKU: CD01509

Size:2 ug

Background:Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated Mouse mast cells, Mouse keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The human IL-3 reported to be a monomer, as it is known, contains 133 amino acids residues which is a single non-glycosylated polypeptide. Specifically, human and Mouse IL-3 share low homology and it does not show activity on Mouse cells.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Interleukin-3

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using human TF-1 cells is less than .1 ng/ml, corresponding to a specific activity of > 1. × 17 IU/mg.

Synonyms:IL-3, IL3, MCGF, MULTI-CSF,Human

Endotoxin:Less than 1 EU/μg of rHuIL-3 as determined by LAL method.

Storage:-20ºC

Sequence:APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF

uniprot accession:P87

Molecular weight:Approximately 15. kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.