Recombinant Human IL-1 Beta protein

SKU: CD01572

Size:2 ug

Background:Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties; The mature human IL-1β shares 96% amino acid sequence identity with rhesus and 67 % -78 % with canine, mouse and rat IL-1β.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Interleukin-1 beta

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse D1S cells is less than 1. pg/ml, corresponding to a specific activity of > 1. × 19 IU/mg.

Synonyms:IL-1β,IL-1, IL1-BETA, IL1F2, IL1beta,IL1B, Human

Endotoxin:Less than 1 EU/μg of rHuIL-1β as determined by LAL method.

Storage:-20ºC

Sequence:APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIP VAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQ FPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS

uniprot accession:P1584

Molecular weight:Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.