Recombinant Human HGPRT/HPRT1 Protein (His Tag)

SKU: PKSH032542

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: HGPRT;HPRT1

Target Synonym: HGPRT;HGPRTase;HPRT;HPRT1;Hypoxanthine-Guanine Phosphoribosyltransferase

Research Areas: Cell biology;epigenetics and nuclear signal

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-His

UNIProt ID: P00492

Accession: P00492

Background: Hypoxanthine-Guanine Phosphoribosyltransferase (HGPRT) has an important role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 functions to salvage purines from degraded DNA to renewed purine synthesis, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Ala218

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, 50% Glycerol, pH8.0.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 27.8 kDa

ObservedMW: 29 kDa