Recombinant Human FGF18 protein (His tag)

SKU: PDEH101057

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:FGF18

Target Synonym:FGFI;zFGF5

Research Areas:Signal Transduction; Stem Cells

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:C-His

UNIProt ID:O76093

Accession:O76093

Background:Fibroblast Growth Factor 18 (FGF-18) is a 20 kDa protein that plays an important role in skeletal development and bone homeostasis . Mature human FGF-18 shares 99% amino acid sequence identity with mouse and rat FGF-18 . It is expressed in embryonic somites and the neural fold , adult lung , cerebellar and hippocampal neurons , hair follicle root sheath cells , and osteogenic mesenchymal cells . FGF-18 binds to FGF R2c, FGF R3c as well as the Golgi protein GLG1 and induces the proliferation of astrocytes and microglia, vascular endothelial cells, dermal fibroblasts, papilla cells, and keratinocytes . FGF-18 is required for normal skeletal development . It recruits osteoclasts and osteoblasts to the growth plate, promotes osteoclast formation and function, inhibits osteoblast differentiation, promotes skeletal vascularization, and induces chondrocyte hypertrophy and cartilage matrix formation.

Concentration:

Activity:Not validated for activity

Sequence:Val 31-Ala 207

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:19.4 kDa

ObservedMW:23 kDa