Recombinant Human FGF-4 protein

SKU: CD01801

Size:5 ug

Background:FGF4, also named FGF-K and K-FGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF-4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF-4 (71-206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with Mouse, rat, canine and bovine FGF-4. Additionally, FGF-4 shares about 30 % sequence identity with the prototypical members of the FGF family.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Fibroblast Growth Factor-4

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than .5 ng/ml, corresponding to a specific activity of > 2. × 16 IU/mg.

Synonyms:FGF-4, FGF4, HBGF-4, HST, HST-1, HSTF-1, HSTF1, K-FGF, KFGFHuman

Endotoxin:Less than 1 EU/μg of rHuFGF-4 as determined by LAL method.

Storage:-20ºC

Sequence:GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL

uniprot accession:P862

Molecular weight:Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 3 mM NaCl.