Recombinant Human EphA3 Protein (His Tag)

SKU: PKSH031116

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: EK4;ETK;ETK1;HEK;HEK4;TYRO4

Research Areas: Signal Transduction;Cardiovascular;Neuroscience;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-His

UNIProt ID: P29320

Accession: NP_005224.2

Background: EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.

Concentration:

Activity: Immobilized human EPHA3-His at 10 μg/ml (100 μl/well) can bind human EphrinA5-Fc, The EC50 of human EphrinA5-Fc is 6. 2-14.6 ng/ml.

Sequence: Met 1-Gln541

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 60.3 kDa

ObservedMW: 65-70 kDa