Recombinant Human EpCAM/TROP-1 Protein (His & Avi Tag)

SKU: PKSH033801

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: EpCAM;TROP-1

Target Synonym: Adenocarcinoma-Associated Antigen;Cell Surface Glycoprotein Trop-1;EGP;EGP314;Ep-CAM;Epithelial Cell Adhesion Molecule;Epithelial Cell Surface Antigen;Epithelial Glycoprotein 314;EpithelialGlycoprotein;KSA;Tumor-Associated Calcium Signal Transduce;hEGP314

Research Areas: Tags & Cell Markers

Conjugation: Biotinylated

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His-Avi

UNIProt ID: P16422

Accession: AAH14785.1

Background: Epithelial Cell Adhesion Molecule (EpCAM) is a signal type I transmembrane glycoprotein that belongs to the EPCAM family. EpCAM is composed of an extracellular domain with one thyroglobulin type-1 domain; a transmembrane domain and a cytoplasmic domain. EpCAM is found on the surface of adenocarcinoma; but not on mesodermal or neural cell membranes. The EpCAM molecule has been shown to function as a homophilic Ca2+ independent adhesion molecule. It may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium as an immunological barrier providing the first line of defense against infection. Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) and diarrhea type 5 (DIAR5). EpCAM plays a role in embryonic stem cells proliferation and differentiation; it up-regulates the expression of FABP5; MYC and Cyclin A and Cyclin E. It is highly and selectively expressed by undifferentiated embryonic stem cells.

Concentration:

Activity: Not validated for activity

Sequence: Gln24-Lys265

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 30.2 kDa

ObservedMW: 35-50 kDa