Recombinant Human DHH C23II protein

SKU: CD01918

Size:5 ug

Background:Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Desert Hedgehog is a secreted, 45 kDa, 373 amino acid protein that undergoes autocatalytic cleavage between Gly198 and Cys199 catalyzed by the C-terminal domain, which releases the N-terminal domain with a concominant attachment of cholesterol at its new C-terminus. Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip). Within the N-terminal domain, human Dhh shares 97% aa sequence identity with mouse and rat Dhh. It shares approximately 75% aa seqeuence identity with human Indian (Ihh) and Sonic Hedgehog (Shh). Dhh is expressed in the female by ovarian granulosa cells and the corporus luteum. Its upregulation in ovarian cancer correlates positively with proliferative index, and negatively with prognosis. Dhh is also expressed by Schwann cells and is upregulated following nerve injury.

Research_area:Others

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Desert Hedgehog Cys23IleIle

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by its ability to induce alkaline phosphatase production by C3H1T1/2(CCL-226) cells is 15-45 μg/ml.

Synonyms:DHH C23II,GDMN, GDXYM, HHG-3, SRXY7, Human

Endotoxin:Less than 1 EU/μg of rHuDHH C23II as determined by LAL method.

Storage:-20ºC

Sequence:IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG

uniprot accession:O43323

Molecular weight:Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 177 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 1mM PB, pH 6., 3mM NaCl.