Recombinant Human CYR61 protein

SKU: CD01947

Size:5 ug

Background:Cysteine-rich angiogenic inducer 61 (Cyr61) encoded by the Cyr61 gene is a dynamically expressed, multifunctional matricellular protein and it is also a secreted, extracellular matrix (ECM)-associated signaling protein of the CCN family. Cyr61 plays essential roles in cardiovascular development during embryogenesis and regulates inflammation, wound healing and fibrogenesis in the adult. Aberrant CCN1 expression is associated with myriad pathologies, including various cancers and diseases associated with chronic inflammation. Mature human Cyr61 shares 93 % amino acid sequence identity with mouse and rat Cyr61. Cyr61 consists of four domains. There are an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Cysteine-rich Angiogenic Inducer 61

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse Balb/3T3 cells is less than 3. μg/ml, corresponding to a specific activity of > 33 IU/mg.

Synonyms:CYR61, CCN1,GIG1, IGFBP1,Human

Endotoxin:Less than 1 EU/μg of rHuCYR61 as determined by LAL method.

Storage:-20ºC

Sequence:TCPAACHCPL EAPKCAPGVG LVRDGCGCCK VCAKQLNEDC SKTQPCDHTK GLECNFGASS TALKGICRAQ SEGRPCEYNS RIYQNGESFQ PNCKHQCTCI DGAVGCIPLC PQELSLPNLG CPNPRLVKVT GQCCEEWVCD EDSIKDPMED QDGLLGKELG FDASEVELTR NNELIAVGKG SSLKRLPVFG MEPRILYNPL QGQKCIVQTT SWSQCSKTCG TGISTRVTND NP

uniprot accession:O622; Q6FI18; O95694;Q9UID7

Molecular weight:Approximately 39.4 kDa, a single non-glycosylated polypeptide chain containing 357 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in citrate buffer solution, 3 mM NaCl, pH 3..