Recombinant Human Cyclophilin C/PPIC Protein (His&Trx Tag)

SKU: PKSH032316

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Cyclophilin C;PPIC

Target Synonym: CYPC;Cyclophilin C;PPIC;PPIase C;Peptidyl-Prolyl Cis-Trans Isomerase C;Rotamase C

Research Areas: Signal Transduction;immunology;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-Trx-His

UNIProt ID: P45877

Accession: P45877

Background: Cyclophilin C is an enzyme (EC 5.2.1.8) found in both prokaryotes and eukaryotes that interconverts the cis and trans isomers of peptide bonds with the amino acid proline. Proline has an unusually conformationally restrained peptide bond due to its cyclic structure with its side chain bonded to its secondary amine nitrogen. Most amino acids have a strong energetic preference for the trans peptide bond conformation due to steric hindrance, but prolines unusual structure stabilizes the cis form so that both isomers are populated under biologically relevant conditions. Proteins with prolyl isomerase activity include cyclophilin, FKBPs, and parvulin, although larger proteins can also contain prolyl isomerase domains.

Concentration:

Activity: Not validated for activity

Sequence: Lys31-Asp182

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 10% Glycerol, pH 7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 33.8 kDa

ObservedMW: 34 kDa