Recombinant Human CTLA-4/CD152 protein (His tag)

SKU: PDMH100155

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Immune Checkpoint Proteins

Abbreviation:CTLA-4;CD152

Target Synonym:CD152;CTLA4;Cytotoxic T-Lymphocyte-Associated Protein 4;Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4

Research Areas:immunology;Stem cells

Conjugation:

Target Species:Human

Expression Host:HEK293 Cells

Application:

Fusion tag:C-His

UNIProt ID:P16410

Accession:P16410

Background:Cytotoxic T-lymphocyte protein 4, is a single-pass type I membrane protein. It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Phe162

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:17.7 kDa

ObservedMW:15 kDa