Recombinant Human CCNA1/Cyclin-A1 Protein (His Tag)

SKU: PKSH031307

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: CT146

Research Areas: Cell biology;Cancer;epigenetics and nuclear signal

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application: ELISA

Fusion tag: N-His

UNIProt ID: P78396

Accession: NP_003905.1

Background: Cyclin A1 is a member of the highly conserved cyclin family that is characterized by a dramatic periodicity in protein abundance, and belongs to the A-type cyclin subfamily. The mammalian A-type cyclin family consists of two members: cyclin A1 and cyclin A2. Different cyclins exhibit distinct expression. Cyclin A1 is expressed in mice exclusively in the germ cell lineage and high rate of cyclinA1 is found in human testis and certain myeloid leukaemia cells. Cyclin A1 is primarily function in the control of meiosis. It serves as regulator subunits binding to cyclin-dependent kinase 1 (Cdk1) and cyclin-dependent kinase 2 (Cdk2), which give two different kinase activities, one appearing in S phase, the other in G2. Through this, cyclin A1 operate the entry and progression in cell cycle. High frequency of cyclin A1 overexpression has been observed in acute myelocytic leukemias, especially those that are at the promyelocyte and myeloblast stages of development.

Concentration:

Activity: 1. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK1. The EC50 of biotinylated human CDK1 is 0.02-0.04 μg/ml. 2. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK2-His. The EC50 of biotinylated human CDK2-His is 0.07-0.15 μg/ml.

Sequence: Met 1-Gln 465

Purity: > 96 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 54.6 kDa

ObservedMW: 50 kDa