Recombinant Human BLK Protein (His Tag)

SKU: PKSH032011

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: BLK

Target Synonym: B Lymphocyte Kinase;BLK;MODY11;Tyrosine-Protein Kinase Blk;p55-Blk

Research Areas: Cell biology

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: C-His

UNIProt ID:

Accession: P51451

Background: Tyrosine-Protein Kinase Blk (BLK) contains one protein kinase domain, one SH2 domain and one SH3 domain. BLK is a non-receptor tyrosine kinase, which is involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulines and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Defects in BLK are a cause of maturity-onset diabetes of the young type 11 (MODY11).

Concentration:

Activity: Not validated for activity

Sequence: Gly2-Pro505

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 1mM DTT, pH 7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 58.7 kDa

ObservedMW: 50-65 kDa