Recombinant Human BD-3 protein

SKU: CD02269

Size:5 ug

Background:Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 k Da proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human β-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because β-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Beta-defensin 3

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by anti-microbial activity against E.coli. is less than 3 μg/ml, corresponding to a specific activity of > 33.3 IU/mg.

Synonyms:BD-3,BD3,DEFB13B, DEFB-3, DEFB13, DEFB3, HBD-3, HBD3, HBP-3, HBP3 Human

Endotoxin:Less than 1 EU/μg of rHuBD-3 as determined by LAL method.

Storage:-20ºC

Sequence:GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK

uniprot accession:P81534

Molecular weight:Approximately 5.2 kDa, a single non-glycosylated polypeptide chain containing 45 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, pH 7.4, 13 mM NaCl.