Recombinant Human 4-1BBL protein

SKU: CD02405

Size:5 ug

Background:4-1BBL is a member of the tumor necrosis factor (TNF) receptor family. This receptor contributes to the clonal expansion, survival, and development of T cells. In addition, 4-1BBL expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. CD137 has been shown to interact with TRAF2. The human 4-1BBL gene codes for a 254 amino acid type II transmembrane containing a 28 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 205 amino acid extracellular domain (ECD). The human 4-1BBL ECD shares 32 % and 35 % a.a. identity with Mouse and rat ECD.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human 4-1BB Ligand

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by stimulation of IL-8 production using human PBMC is less than 1 ng/ml, corresponding to a specific activity of > 1. × 15 IU/mg.

Synonyms:TNFSF9,4-1BBL, 4-1BB-L, CD137L, TNLG5A,Human

Endotoxin:Less than 1 EU/μg of rHu4-1BBL as determined by LAL method.

Storage:-20ºC

Sequence:REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS PRSE

uniprot accession:P41273

Molecular weight:Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 184 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.