Recombinant Horse Troponin I, cardiac muscle (TNNI3)

SKU: Q5PYI0

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Cardiovascular

Uniprot ID: Q5PYI0

Gene Names: TNNI3

Alternative Name(s): (Cardiac troponin I)

Abbreviation: Recombinant Horse TNNI3 protein

Organism: Equus caballus (Horse)

Source: E.coli

Expression Region: 2-205aa

Protein Length: Full Length of Mature Protein

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein Sequence: ADQSGNAAPPPVRRRSSANYRAYATEPHAKKKSKISASRKLQLKTLMLQIAKQELEREAVERRGEKGRALSTRCQPLELAGLGFEELQDLCRQLHARVDKVDEERYDVEAKVTKNITEIADLNQKIFDLRGKFKRPTLRRVRISADAMMQALLGTRAKETLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFEG

MW: 30.8 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Reference: "Cloning and sequencing of equine cardiac troponin I and confirmation of its usefulness as a target analyte for commercial troponin I analyzers." Rishniw M., Simpson K.W. J. Vet. Diagn. Invest. 17: 582-584(2005)

Function: