Recombinant Hirudin protein

SKU: CD02411

Size:2 ug

Background:Hirudin is the most potent natural thrombin-specific protease inhibitor, and is originally derived in the salivary glands of the medicinal leech. Unlike heparin, hirudin act directly on thrombin, rather than through other clotting factors. They have a high binding affinity and specificity for thrombin. Therefore, hirudin prevents or dissolves the formation of clots and thrombi, and has therapeutic value in blood coagulation disorders, in the treatment of skin hematomas and of superficial varicose veins, either as an injectable or a topical application cream.

Research_area:Cardiovascular

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Pichia Pastoris

Full product name:Recombinant Hirudin

Biological activity:The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 14, ATU/mg protein.

Synonyms:Hirudin, Recombinant

Endotoxin:Less than 1 EU/μg of rHirudin as determined by LAL method.

Storage:-20ºC

Sequence:VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EYL

uniprot accession:P15

Molecular weight:Approximately 6.7 kDa, a single non-glycosylated polypeptide chain containing 63 amino acid residues.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution of 2 mM PBS, pH 7., containing 2 % mannitol.