Recombinant Danio rerio U11/U12 small nuclear ribonucleoprotein 35 kDa protein (snrnp35)

SKU: Q4KMD3

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: Q4KMD3

Gene Names: snrnp35

Alternative Name(s): (U11/U12 snRNP 35 kDa protein)(U1 snRNP-binding protein homolog)

Abbreviation: Recombinant Zebrafish snrnp35 protein

Organism: Danio rerio (Zebrafish) (Brachydanio rerio)

Source: E.coli

Expression Region: 1-208aa

Protein Length: Full Length

Tag Info: N-terminal 6xHis-tagged

Target Protein Sequence: MEWSPVAKVYDPLKAGSIDGTDVEPHDAGVWRAMLARYKPNRGVCGDPDLTLFVARLNPQTTEEKLRDVFSKFGDIRRLRLVRDVVTGFSKRYAFIEYKEERSLKRAWRDANKLILDQYELLVDVEQERTLPGWRPRRLGGGQGGQKESGQLRFGGRDRPFRKPINLSTRRPAEPRGRETERERDRRDYRDRRHERTHTEDRTHRHTY

MW: 28.7 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor.

Reference: "Cloning of cDNA and high-level expression of equine vascular endotherial growth factor (VEGF)." Miura N., Misumi K., Kawahara K., Nakashima M., Fukumitsu S., Kawabata H., Uto N., Oka T., Maruyama I., Sakamoto H. Submitted (JAN-2001)

Function: