Recombinant Chicken Growth/differentiation factor 9 (GDF-9)

SKU: Q6Q247

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: Q6Q247

Gene Names: GDF-9

Alternative Name(s):

Abbreviation: Recombinant Chicken GDF-9 protein

Organism: Gallus gallus (Chicken)

Source: E.coli

Expression Region: 25-454aa

Protein Length: Full Length of Mature Protein

Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein Sequence: SPRSRDRTAPDKVSGLLGAPELHPLLRLPKGVSRGYALLPPLLEVLSDQGRQSWESGTPRLQPDSRALRYMKRLYKMYATKEGIPKAHKSHLYNTVRLFTPCSECQHRHRDPITGDFHSVDLLFNLDRVTALEHLLKSVLLYSFDTSVPTSSFTCTCHLSVKEHDFSSQVCPSISHSVAFSLHFEVRKRKWVEIDVTSFLRPLIATNRRNIHMAVNFTCLMGNPQHNTKQDNLINVALVPPSLLLYLNDTSEQAYHRWNSLRHRRKSPVRPKQRSSLFADVTGDEGRQNTQGKRASRHRREENLKEAPATAPQNLSEYFKQFLFPQNECELHSFRLSFSQLKWDKWIIAPHRYSPQYCKGDCPRVVGHRYGSPVHTMVQNIIYEKLDSSVPRPSCVPAEYSPLSVLTIEPDGSIVYKEYEDMIATKCTCR

MW: 56.8 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Plays an important role in the final stage of carbohydrate digestion. Isomaltase activity is specific for both alpha-1,4- and alpha-1,6-oligosaccharides.

Reference: "Structural basis for substrate selectivity in human maltase-glucoamylase and sucrase-isomaltase N-terminal domains." Sim L., Willemsma C., Mohan S., Naim H.Y., Pinto B.M., Rose D.R. J. Biol. Chem. 285: 17763-17770(2010)

Function: