Recombinant Cdc25C Monoclonal Antibody

SKU: AN301883L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:Cdc25C

Target Synonym:CDC;PPP1R;CDC25C;CDC25;PPP1R60

Research Areas:Cell Biology;Epigenetics and Nuclear Signaling

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A595

UNIProt ID:P30307

Accession:

Background:Cdc25C is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis. Cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint. When phosphorylated at Ser216, Cdc25C binds to members of the 14-3-3 family of proteins, sequestering Cdc25C in the cytoplasm and thereby preventing premature mitosis. The checkpoint kinases Chk1 and Chk2 phosphorylate Cdc25C at Ser216 in response to DNA damage.

Concentration:1 mg/mL

Immunogen:Recombinant human Cdc25C fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:2000

Calculated MW:53 kDa

ObservedMW:53 kDa