Recombinant CD3D Monoclonal Antibody

SKU: AN301813L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:CD3D

Target Synonym:IMD;CD3D;CD3-DELTA;IMD19;T3D;T-Cell Receptor T3 Delta Chain;T-cell surface glycoprotein CD3 delta chain;T3;CD3 delta

Research Areas:Immunology;Stem Cells

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;

Application:WB;IHC;IF;FCM;IP

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A525

UNIProt ID:P04234

Accession:

Background:CD3D is a component of the TCR-CD3 complex, which is present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition, CD3D plays an essential role in thymocyte differentiation. It participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. It interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.

Concentration:1 mg/mL

Immunogen:Recombinant human CD3D fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:2000-1:10000;IHC 1:200-1:1000;IF 1:50-1:200;FCM 1:50-1:100;IP 1:25-1:50

Calculated MW:19 kDa

ObservedMW:23 kDa