Recombinant APAF1 Monoclonal Antibody

SKU: AN301989L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:APAF1

Target Synonym:KIAA;Apoptotic protease-activating factor;Apoptotic peptidase activating factor;CED;APAF1;APAF-1;CED4;APAF;APAF 1;Apoptotic peptidase activating factor 1;Apoptotic protease activating factor;Apoptotic protease activating factor 1;Apoptotic protease-activating factor 1;CED 4;KIAA0413

Research Areas:Cell Biology;Cancer;Metabolism

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A709

UNIProt ID:O14727

Accession:

Background:Apoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the C. elegans apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis. Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3. The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat. Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli.

Concentration:1 mg/mL

Immunogen:Peptide. This information is proprietary to PTMab.

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:1000-1:3000;IHC 1:100

Calculated MW:142 kDa

ObservedMW:140 kDa