Rat TSP-1(Thrombospondin-1) Microsample ELISA Kit

SKU: ELK10312MS

Size:96T

Reactivity:Rat

Alternative Names:TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1

Assay Type:Sandwich

Sensitivity:0.47 ng/mL

Standard:100 ng/mL

Detection range:1.56-100 ng/mL

Sample type:Plasma

Assay length:3.5h

Research Area:Infection immunity;Hematology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q71SA3