Rat Transmembrane Glycoprotein Nmb, GPNMB ELISA Kit

SKU: E2293Ra

Size:96 Tests-1 Kit

Background:Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. Two transcript variants encoding 560 and 572 amino acid isoforms have been characterized for this gene in humans. The mouse and rat orthologues of GPNMB are known as DC-HIL and Osteoactivin (OA), respectively. GPNMB is a type I transmembrane glycoprotein which shows homology to the pmel17 precursor, a melanocyte-specific protein. In osteoblast progenitor cells, Osteoactivin works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization [9] that is mediated at least in part by Bone morphogenetic protein 2 in a SMAD1 dependent manner to promote osteoblast differentiation. In addition, using a rat fracture model, Osteoactivin (OA) enhances the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, Osteoactivin expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.

Research_area:Immunology

Applications:ELISA

Target_protein:GPNMB

Reactivity:Rat

Assay type:Sandwich

Sensitivity:1.63ng/L

Detection range:3-900ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Transmembrane glycoprotein NMB

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q6P7C7

MASS(Da):63,731

GeneID:113955

Synonyms:GPNMB;Transmembrane glycoprotein NMB

Gene names:Gpnmb

Standard solution:960ng/L