Rat TNNT1(Troponin T Type 1, Slow Skeletal) Microsample ELISA Kit

SKU: ELK6911MS

Size:96T

Reactivity:Rat

Alternative Names:ANM; STNT; TNT; TNTS; Slow Skeletal Muscle Troponin T

Assay Type:Sandwich

Sensitivity:5.3 pg/mL

Standard:1000 pg/mL

Detection range:15.63-1000 pg/mL

Sample type:Serum, plasma and other biological fluids.

Assay length:3.5h

Research Area:Cardiovascular biology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNNT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNNT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNNT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNNT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q7TNB2