Rat SOX9(Sex Determining Region Y Box Protein 9) ELISA Kit

SKU: ELK11743

Size:96T

Reactivity:Rat

Alternative Names:CMPD1; CMD1; SRA1; Campomelic Dysplasia,Autosomal Sex-Reversal; Transcription factor SOX-9

Assay Type:Sandwich

Sensitivity:6.1 pg/mL

Standard:1000 pg/mL

Detection range:15.63-1000 pg/mL

Sample type:tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:Reproductive science;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SOX9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SOX9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SOX9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SOX9 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:F1LYL9