Rat Pyruvate Dehydrogenase Kinase Isoform 4,PDK4 ELISA KIT

SKU: E2100Ra

Size:96 Tests-1 Kit

Background:Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism. {ECO:0000269|PubMed:11486000}.

Research_area:

Applications:ELISA

Target_protein:Pdk4

Reactivity:Rat

Assay type:Sandwich

Sensitivity:0.023ng/ml

Detection range:0.05-20ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Pyruvate Dehydrogenase Kinase Isoform 4

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:O54937

MASS(Da):46679

GeneID:89813

Synonyms:Pdk4

Gene names:Pdk4

Standard solution:24ng/ml