Rat PSA(ProstateSpecific Antigen) Microsample ELISA Kit

SKU: ELK9631MS

Size:96T

Reactivity:Rat

Alternative Names:KLK3; APS; KLK2A1; hK3; Kallikrein-3; Kallikrein-Related Peptidase 3; Gamma-seminoprotein

Assay Type:Sandwich

Sensitivity:0.1 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Tags & Cell Markers;Neuroscience;Signal Transduction;Cancer

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PSA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PSA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PSA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PSA in the samples is then determined by comparing the OD of the samples to the standard curve.

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