Rat GnRH(Gonadotropin Releasing Hormone) ELISA Kit

SKU: ELK5453

Size:96T

Reactivity:Rat

Alternative Names:GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1

Assay Type:Competitive Inhibition

Sensitivity:5.13 pg/mL

Standard:1000 pg/mL

Detection range:15.63-1000 pg/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Endocrinology;Reproductive science;Neuro science;Genetic science;Hormone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GnRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P07490