Rat GDF2(Growth Differentiation Factor 2) Microsample ELISA Kit

SKU: ELK6664MS

Size:96T

Reactivity:Rat

Alternative Names:BMP9; Bone Morphogenetic Protein 9

Assay Type:Sandwich

Sensitivity:0.06 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Cytokine;Metabolic pathway;Developmental science;Bone metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GDF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GDF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GDF2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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