Rat GC(Glucagon) ELISA Kit

SKU: ELK6267

Size:96T

Reactivity:Rat

Alternative Names:GCG; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone

Assay Type:Competitive Inhibition

Sensitivity:8.99 pg/mL

Standard:1500 pg/mL

Detection range:23.44-1500 pg/mL

Sample type:Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Endocrinology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GC in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P06883