Rat COPT1(Copper Transporter 1) ELISA Kit

SKU: ELK11313

Size:96T

Reactivity:Rat

Alternative Names:SLC31A1; CTR1; hCTR1; High Affinity Copper Uptake Protein 1; Solute Carrier Family 31 Member 1

Assay Type:Sandwich

Sensitivity:0.132 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Metabolic pathway;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat COPT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat COPT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat COPT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat COPT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q9JK41