Rad17 (phospho Ser646) rabbit pAb

SKU: ES1536

Size: 100μL

Source:Rabbit

Applications:WB; ELISA;IHC

Reactivity:Human;Mouse;Rat

Dilution:WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000

Immunogen:Synthesized phospho-peptide around the phosphorylation site of human Rad17 (phospho Ser646)

Storage_stability:-20°C/1 year

Clonality:Polyclonal

Isotype:IgG

Concentration:1 mg/ml

Observed_band(KD):

Human_gene_id:5884

Human_swiss_prot_no:O75943

Subcellular_location:Nucleus . Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.

Other_name:RAD17; R24L; Cell cycle checkpoint protein RAD17; hRad17; RF-C/activator 1 homolog

Background:The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, h