QA ELISA kit (General species)

SKU: CEK552Ge

Size: 96Tests

# of Times Cited in literature: 11

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: QA

Target Full Name: Quinolinic Acid

Alternative Names: QUIN; Pyridine-2,3-Dicarboxylic Acid

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with General species QA / Quinolinic Acid

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 1.23-100ng/mL

Sensitivity: 0.60ng/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Quinolinic Acid (QA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Quinolinic Acid (QA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Quinolinic Acid (QA). No significant cross-reactivity or interference between Quinolinic Acid (QA) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Quinolinic Acid (QA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Quinolinic Acid (QA) and unlabeled Quinolinic Acid (QA) (Standards or samples) with the pre-coated antibody specific to Quinolinic Acid (QA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Quinolinic Acid (QA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Quinolinic Acid (QA) in the sample.

Research Area: -

References Citing This Product: M30 Antagonizes Indoleamine 2,3-Dioxygenase Activation and Neurodegeneration Induced by Corticosterone in the Hippocampus.

Monoamine oxidase A upregulated by chronic intermittent hypoxia activates indoleamine 2, 3-dioxygenase and neurodegeneration

Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats

Exercise training impacts skeletal muscle gene expression related to the kynurenine pathway

Serotonin is the main tryptophan metabolite associated with psychiatric comorbidity in abstinent cocaine-addicted patients

Kynurenine 3-monooxygenase upregulates pluripotent genes through β-catenin and promotes triple-negative breast cancer progression