Purified Rabbit anti-ZNF346 Antibody

SKU: DL98215A

Size 1: 100 ul

Synonyms : JAZ; Zinc finger protein 346; Just another zinc finger protein

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to ZNF346

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human ZNF346

WB description: Western blot analysis of ZNF346 expression in A549 (A), MCF7 (B), mouse brain (C) whole cell lysates.

IHC description: Immunohistochemical analysis of ZNF346 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of ZNF346 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 23567

Gene ID (Mouse): 26919

Gene ID(Rat):

SwissProt (Human): Q9UL40

SwissProt (Mouse): Q9R0B7

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.