Purified Rabbit anti-TOPBP1 Antibody

SKU: DL97969A

Size 1: 100 ul

Synonyms : KIAA0259; DNA topoisomerase 2-binding protein 1; DNA topoisomerase II-beta-binding protein 1; TopBP1; DNA topoisomerase II-binding protein 1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to TOPBP1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human TOPBP1

WB description: Western blot analysis of TOPBP1 expression in HEK293T (A) whole cell lysates.

IHC description: Immunohistochemical analysis of TOPBP1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of TOPBP1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 11073

Gene ID (Mouse): 235559

Gene ID(Rat):

SwissProt (Human): Q92547

SwissProt (Mouse): Q6ZQF0

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.